Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Met

来源 :高等学校化学研究：英文版 | 被引量 : 0次 | 上传用户：packey80

【摘要】

：

According to the amino acid sequence and codon preference of E. coli,the human interleukin-18(IL-18) gene was optimized to avoid the rare codons. The total leng

【作者】

：

GAO Chao-hui SHI Xiao-yue HOU

【机构】

：

CollegeofLifeScience,KeyLaboratoryforMolecularEnzymologyandEngineeringofMinistryofEducation,Changehu

【出处】

：

高等学校化学研究：英文版

【发表日期】

：

2008年4期

【关键词】

：

IL-18基因基因合成一步法密码子最优化 Human IL-18 Gene synthesis One-step method Oligodeoxynuc

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According to the amino acid sequence and codon preference of E. coli,the human interleukin-18(IL-18) gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides,DNA fragments were designed and synth

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Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Met

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