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目的：观察三七总皂甙（ＰＮＳ）对血清和肺组织血管紧张素转换酶（ＡＣＥ）的影响。方法：①ＰＮＳ与马尿酰甘氨酰甘氨酸和血清或肺组织匀浆共浴；②静脉注射ＰＮＳ，应用紫外分光光度法测定马尿酸产出率，以其代表ＡＣＥ活性。结果：离体反应显示，随着ＰＮＳ浓度增高（０．１５～１．２ｍｇ／ｍｌ），大白鼠血清、肺组织和人血清ＡＣＥ活性逐渐下降（Ｐ＜０．０１）。ＰＮＳ为１．２ｍｇ／ｍｌ时，抑制率分别为（６１．５４±１１．７６）％、（７２．５４±７．８３）％和（６２．０９±１６．４）％，三者之间无显著性差异（Ｐ＞０．０５）。ＰＮＳ（７５ｍｇ／ｋｇ）静脉注射家兔血清ＡＣＥ活性从（６２．５２±１２．４３）ＩＵ下降为（４４．８０±１０．４７）ＩＵ（Ｐ＜０．００１）。结论：ＰＮＳ对循环和局部组织ＡＣＥ活性均有抑制作用，其抑制原理可能与人工合成ＡＣＥ抑制剂不同
Objective: To observe the effect of panax notoginseng saponins (PNS) on serum angiotensin-converting enzyme (ACE) in lung tissue. METHODS: 1PNS was co-bathed with hipylated glycylglycine and serum or lung homogenate; 2 intravenous injection of PNS, UV spectrophotometry was used to determine hippocampal acid production rate, which represented ACE activity. RESULTS: The ex vivo reaction showed that with the increase of PNS concentration (0.15-1.2 mg/ml), serum ACE activity in serum, lung tissue, and human serum gradually decreased (P<0.01). When PNS was 1.2 mg/ml, the inhibition rates were (61.54±11.76)%, (72.54±7.83)%, and (62.09±16.4)%, respectively. No significant difference (P>0.05). The serum ACE activity of rabbits injected with PNS (75mg/kg) decreased from (62.52±12.43)IU to (44.80±10.47)IU (P<0.001). Conclusion: PNS has inhibitory effects on circulatory and local ACE activity. The principle of inhibition may be different from synthetic ACE inhibitors.