PROTEOME ANALYSIS OF RICE ROOT PROTEINS REGULATED BY GIBBERELLIN

来源 :Genomics Proteomics & Bioinformatics | 被引量 : 0次 | 上传用户:zb272939419
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To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, e?ective, and reliable tool in di?erential protein expressions. In previous studies, sixteen proteins showed di?erences in accumulation levels as a result of treatment with GA3, uniconazole, or abscisic acid (ABA), and/or the di?erences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA3, was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth. To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, e? Ective, and reliable tool in erential protein expressions. In previous studies, sixteen proteins showed di? erences in accumulation levels as a result of treatment with GA3, uniconazole, or abscisic acid (ABA), and / or the di erences between the GA-deficient semi- Among these proteins, aldolase increased in roots treated with GA3, was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced gr owth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth.
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