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目的通过对小儿腹泻患者的粪便进行轮状病毒(Rotavirus,RV)的检测,探讨不同季节、不同年龄段小儿腹泻患者与轮状病毒感染的相关性,为小儿腹泻病因的诊断提供一种手段,帮助更好的诊断、治疗腹泻。方法收集2011年7-12月中南大学湘雅医院儿科门诊初步诊断为腹泻的患儿(0~5岁)粪便标本(139例),采用A群轮状病毒诊断试剂盒检测样本中A群轮状病毒抗原。结果 139例腹泻患儿粪便,35例轮状病毒抗原检测阳性,阳性率为25.18%;其中7-8月份阳性率为21.79%(17/78),9-10月份阳性率为22.58%(7/31),11-12月份阳性率为46.67%(14/30);0~1岁患儿阳性率为28.41%(25/88),1~2岁患儿阳性率35.29%(12/34),2~5岁患儿阳性率为5.88%(1/17);男性患儿阳性率23.26%(20/86),女性患儿阳性率33.96%(18/53)。结论 A群RV引起的婴幼儿急性腹泻主要集中在秋冬季,1~2岁的婴幼儿为A群RV感染的高峰期。男女婴幼儿间的感染率差异无统计学意义(P>0.05)。
Objective To detect Rotavirus (RV) in the stool of children with diarrhea and explore the correlation between rotavirus infection and children with diarrhea in different seasons and different ages so as to provide a means of diagnosing the cause of pediatric diarrhea, Help better diagnosis and treatment of diarrhea. Methods A total of 139 stool specimens from children (0-5 years old) with pediatric outpatients diagnosed as diarrhea in Xiangya Hospital of Central South University from July to December 2011 were collected. Group A rotavirus diagnostic kit Like virus antigen. Results The positive rate of urine samples from 139 cases of diarrhea and 35 rotavirus antigens was 25.18%. The positive rate was 21.79% (17/78) in July-August, and the positive rate was 22.58% (7.58%) in July-August / 31). The positive rate was 46.67% (11/30) in 11-12 months. The positive rate was 28.41% (25/88) in children aged 0-1 and 35.29% (1-12) ). The positive rate was 5.88% (1/17) in children aged 2 ~ 5 years. The positive rate was 23.26% (20/86) in males and 33.96% (18/53) in females. Conclusion A group of RV-induced acute diarrhea in infants and young children mainly concentrated in the autumn and winter, infants and young children aged 1-2 years as the peak of group A RV infection. There was no significant difference in infection rates between boys and girls (P> 0.05).