从粪便中提纯甲型肝炎病毒的方法有多种,本文介绍用免疫亲和层析法从粪便中浓缩和提纯甲型肝炎病毒的简单,快速方法。所用IgG是从第一次在粪便中发现甲型肝炎病毒后10周的恢复期病人血清中提纯的。用溴化氰活化了的琼脂糖珠4B1.2毫升与30毫克的IgG混合,89%的免疫球蛋白可偶联到此琼脂糖珠4B上,将其装入有直径10毫米熔结玻璃底板的层析柱。用含有0.02%叠氮钠的PBS洗涤和平衡。含有甲型肝炎 There are several ways to purify the hepatitis A virus from the excrement. This article describes a simple, rapid method of concentrating and purifying the Hepatitis A virus from the stool using immunoaffinity chromatography. The IgG used was purified from the patient’s sera 10 weeks after the first recovery of Hepatitis A virus in the stool. Sepharose 5B1 activated with cyanogen bromide 4B1.2 ml was mixed with 30 mg of IgG and 89% of the immunoglobulins could be coupled to this agarose bead 4B and loaded into a frit glass substrate 10 mm in diameter Of the column. Wash and equilibrate with PBS containing 0.02% sodium azide. Contains Hepatitis A