目前对决定HCV感染预后的因素仍缺乏了解,作者对10例因产后注射被污染的抗-D免疫球蛋白而感染1b型HCV的患者,针对核心蛋白诱导的CD4T细胞反应进行了分析。 这10例慢性感染者的HCV核心蛋白基因序列间的差异很小(0.1％),且高度保守。以共同序列合成多肽,在体外用多肽刺激HCV感染者的外周血单个核细胞(PBMC),PBMC得以增殖,并有针对四个HCV核心蛋白免疫显性区域的IFN-γ产生。四个免 Currently, there is still a lack of understanding of the factors that determine the prognosis of HCV infection. The authors analyzed core protein-induced CD4 T cell responses in 10 patients infected with type 1b HCV due to post-natal injections of contaminated anti-D immunoglobulin. The differences between the HCV core protein gene sequences in these 10 chronic infections were small (0.1%) and highly conserved. The polypeptides were synthesized in a common sequence and the PBMCs were stimulated with the polypeptide in vitro to proliferate PBMCs and to have IFN-gamma production against immunodominant regions of the four HCV core proteins. Four free