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在 5 3只麻醉Sprague Dawley大鼠观察了最后区内微量注射腺苷 (1ng/ 6 0nl)对平均动脉压 (MAP)、心率(HR)和肾交感神经放电 (RSNA)的影响。实验结果如下 :(1)最后区内微量注射Ado后 ,MAP、HR和RSNA分别由13 76± 0 46kPa、35 6 2 8± 4 2 5bpm和 10 0± 0 %下降至 11 2 3± 0 49kPa (P <0 0 0 1)、336 91± 5 2 3bpm (P <0 0 1)和70 95± 5 19% (P <0 0 0 1) ;(2 )静脉注射非选择性腺苷受体拮抗剂 8 苯茶碱 (8 phenyltheophylline,15 0 μg/kg ,0 2ml)和选择性腺苷A1受体拮抗剂 (8 cyclopentyl 1,3 dipropylxanthine,5 0 0 μg /kg ,0 2ml)后 ,腺苷的上述抑制效应可被完全阻断 ;(3)静脉注射ATP敏感性钾通道阻断剂格列苯脲 (5mg/kg ,0 2ml)后 ,腺苷的上述效应也被消除。以上结果提示 ,最后区微量注射腺苷对血压、心率和肾交感神经放电有抑制作用 ,此作用与A1受体介导的ATP敏感性钾通道开放有关。
In 53 anesthetized Sprague Dawley rats, the effects of microinjection of adenosine (1ng / 600nl) into the final zone on mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve discharge (RSNA) were observed. The results are as follows: (1) MAP, HR and RSNA decreased from 13 76 ± 0 46 kPa, 35 6 2 8 ± 4 2 5 bpm and 10 0 ± 0% to 11 2 3 ± 0 49 kPa (P <0.01), 336 91 ± 5 2 3bpm (P <0.01) and 70 95 ± 5 19% (P <0.01). (2) Non-selective adenosine receptor antagonist After administration of 8 phenyltheophylline (15 0 μg / kg, 0 2 ml) and selective 8-cyclopentyl 1,3-dipropylxanthine (500 μg / kg, 0 2 ml) The above inhibitory effect can be completely blocked; (3) The above effects of adenosine are also eliminated after iv injection of glibenclamide (5 mg / kg, 0 2 ml) as an ATP-sensitive potassium channel blocker. These results suggest that the last zone of microinjection of adenosine on blood pressure, heart rate and renal sympathetic nerve discharge inhibition, the role and A1 receptor-mediated ATP-sensitive potassium channel opening.