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为深入研究人Ⅰ型免疫缺陷病毒（ＨＩＶ—１）衣壳蛋白ｐ２４的结构与功能，制备抗Ｐ２４单克隆抗体及其诊断抗原，将编码ＨＩＶ－１ｐ２４蛋白的ｐ２４ｇａｇ基因片段，克隆到原核表达载体ｐＥＴ－１７ｂ的Ｔ７噬菌体启动子下游，构建成了重组表达质粒ｐＥＴ２４，并使ｐ２４ｇａｇ基因片段在大肠杆菌ＢＬ２１（ＤＥ３）中高效表达，其产物为３０ｋＤａ的ｓ１０－ｐ２４融合蛋白，表达量占菌体总蛋白的３８．４％。重组Ｐ２４蛋白均抗ｐ２４单克隆抗体及ＨＩＶ—１阳性血清发生特异性反应，具有较好的抗原性，对研究开发ＡＩＤＳ诊断试剂和基因工程疫苗具有重要意义。
In order to further study the structure and function of capsid protein p24 of human immunodeficiency virus type 1 (HIV-1), an anti-P24 monoclonal antibody and its diagnostic antigen were prepared. The p24gag gene fragment encoding HIV-1 p24 protein was cloned into the prokaryotic expression vector pET-17b downstream of the T7 phage promoter to construct recombinant expression plasmid pET24, and p24gag gene fragment was highly expressed in E. coli BL21 (DE3), and its product was 30kDa s10-p24 fusion protein, 38.4% of total protein. Recombinant P24 protein has specific anti-p24 monoclonal antibody and HIV-1 positive serum reaction, has good antigenicity, research and development of AIDS diagnostic reagents and genetic engineering vaccine is of great significance.