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目的 了解 1999年 11月～ 2 0 0 0年 1月我国河南省汝阳县婴幼儿流行性喘憋性肺炎(简称流喘肺炎 )暴发流行的病原。方法 分别采集 12例流喘肺炎患儿的咽拭子和鼻咽分泌物标本进行病毒分离、间接免疫荧光检测常见的呼吸道病毒抗原以进行快速诊断 ;用RT PCR对分离到的呼吸道合胞病毒 (RSV)株进行分型 ;16例用免疫荧光法检测双份血清中RSV抗体滴度 ;用核苷酸序列测定对分离到的病毒株进行基因分析。结果 12例患儿咽拭子标本中 (除 1例污染外 ) 4例分离到RSV ,阳性率为 36 % ,3例为A亚型 ,1例为B亚型 ;12例鼻咽分泌物脱落细胞经免疫荧光法检测 9例为RSV阳性 ,阳性率为 75 % ,未检测到其它呼吸道病毒 ;16例患儿双份血清中 ,有 10例 (6 3 % )恢复期血清RSV特异性IgG抗体比急性期有 4倍以上升高 ,分离株F蛋白基因的部分序列测定显示与RSV的A亚型标准株有较高的同源性。结论 此次婴幼儿流喘肺炎流行的病原主要为A亚型RSV
Objective To understand the prevalence of outbreaks of epidemic pneumonia in infants and young children in Ruyang County, Henan Province from November 1999 to January 2000. Methods Throat swabs and nasopharyngeal secretions from 12 children with asthmatic pneumonia were collected for virus isolation. Indirect immunofluorescence was used to detect common respiratory virus antigens for rapid diagnosis. Respiratory syncytial virus RSV) strains were screened. Twenty-six anti-RSV antibody titers were detected by immunofluorescence in two serum samples. The nucleotide sequences were used to analyze the isolated virus strains. Results The positive rate of RSV was isolated in 4 of 12 children with throat swabs (except 1 case of contamination), the positive rate was 36%, 3 cases were subtype A and 1 case was subtype B. 12 cases of nasopharyngeal secretions shed Nine of the cells were positive for RSV by immunofluorescence assay and the positive rate was 75%. No other respiratory viruses were detected. Serum RSV specific IgG antibody was recovered in 10 of 16 patients (6 3%) Which was more than 4 times higher than that in acute phase. The partial sequence analysis of the F protein gene of the isolate showed high homology with the standard subtype A of RSV. Conclusion The prevalence of asthmatic pneumonia in infants and young children is mainly subtype A RSV