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目的:观察黄芪甲苷对高浓度皮质酮损伤海马神经细胞活性及学习记忆相关蛋白GCR及SYN1表达的影响。方法:采用CCK8法,FDA/PI双荧光染色观察皮质酮对原代培养新生24h大鼠海马神经细胞存活率的影响,同时观察黄芪甲苷的保护作用;利用实时定量PCR及Western blot法检测黄芪甲苷对皮质酮损伤细胞GCR和SYN1 mRNA及蛋白表达的影响。结果:与对照组比,皮质酮能够时间剂量依赖性的降低神经细胞存活率,而0.1μmol/L、1μmol/L及10μmol/L黄芪甲苷及10μmol/L美金刚均能不同程度地提高损伤细胞的存活率;1μmol/L黄芪甲苷能上调损伤细胞GCR和SYN1 mRNA及蛋白表达。结论:黄芪甲苷对高浓度皮质酮损伤神经细胞具有保护作用,并能够上调学习记忆相关蛋白GCR和SYN1的表达。
AIM: To observe the effects of astragaloside on the activity of hippocampal neurons and the expression of learning and memory related proteins (GCR and SYN1) induced by high concentrations of corticosterone. Methods: CCK8 assay and FDA / PI double staining were used to observe the effect of corticosterone on the survival rate of primary cultured neonatal rat hippocampal neurons 24 h. Meanwhile, the protective effect of astragaloside Ⅳ was observed. Real-time quantitative PCR and Western blot were used to detect the effects of astragalus Effect of methyl glycoside on the expression of GCR and SYN1 mRNA and protein in corticosterone - induced injury. Results: Compared with the control group, corticosterone reduced the survival rate of neurons in a dose-dependent manner in a dose-dependent manner. However, 0.1μmol / L, 1μmol / L and 10μmol / L astragaloside and 10μmol / Cell survival rate; 1μmol / L Astragaloside could up-regulate GCR and SYN1 mRNA and protein expression in injured cells. CONCLUSION: Astragaloside has a protective effect on high concentration corticosterone-injured neurons and can up-regulate the expression of learning and memory-related proteins GCR and SYN1.