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从广东省阳江县的土壤中,分离到一种病原斯氏线虫(Steinernema sp.8503),从线虫3龄幼虫体,又分离出8503菌株。此菌为共生菌,革兰氏阴性、非孢子型杆菌,2.0～12.0×0.6～1.3微米,鞭毛为周毛,硝酸盐不产生亚硝酸盐,过氧化氢酶阴性,脲素酶阴性,磷酸脂酶阴性,卵磷酯酶阳性,七叶苷水解阳性,不具荧光,对昆虫有致病性,从一系列的生化特性和线虫的带菌率、细菌囊和细菌的形状、分布等检查,此菌为大肠杆菌科的Xenorhabdus nemalophilus。对促进线虫在人工培养基的繁殖,提高线虫的产量和毒力均有一定的价值。
A strain of Steinernema sp. 8503 was isolated from the soil of Yangjiang County, Guangdong Province. From the third instar larvae of C. elegans, 8503 strains were isolated. The bacteria is a symbiotic bacteria, Gram-negative, non-spore bacilli, 2.0 ~ 12.0 × 0.6 ~ 1.3 microns, flagella pericytes, nitrite does not produce nitrite, catalase negative, urease negative, phosphoric acid Lipase-negative, lecithinase-positive, phyllosinic hydrolyzed positive, non-fluorescent, pathogenic to insects, from a series of biochemical characteristics and nematode carrier rate, bacterial sac and bacterial shape, distribution and other checks, The bacterium is Xenorhabdus nemalophilus of the Escherichia coli family. To promote the growth of nematode in artificial media, increase the production and virulence of nematodes have a certain value.