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目的通过检测肺炎支原体肺炎(MPP)患儿喘息组、非喘息组和正常对照组儿童血清白介素-33(IL-33)的水平,以探讨IL-33在MPP发病过程中的变化及意义。方法选取2013年6月—2014年8月间丽水市人民医院确诊为MPP的45例住院患儿作为观察对象。根据患儿病程中有无合并喘息和(或)气急症状及肺部闻及哮鸣音分为喘息组和非喘息组,其中喘息组15例、非喘息组30例。同期收集门诊健康体检儿童25例作为正常对照组。采用双抗体夹心酶联免疫吸附法(ELISA)检测MPP各组治疗前、治疗后及正常对照组血清IL-33的水平。结果喘息组和非喘息组治疗前血清IL-33水平均升高,与正常对照组比较,差异有统计学意义(P<0.05);治疗后,喘息组血清IL-33水平高于正常对照组,差异有统计学意义(P<0.05),非喘息组血清IL-33水平与正常对照组比较,差异无统计学意义(P>0.05)。喘息组与非喘息组比较,治疗前2组血清IL-33水平均升高,差异无统计学意义(P>0.05);治疗后,喘息组血清IL-33水平仍高于非喘息组,2组间差异有统计学意义(P<0.05)。结论IL-33可能参与MPP的免疫炎症反应,MPP伴喘息者血清IL-33表达时间较MPP非喘息者长。对MP感染伴喘息患儿要高度重视,早期进行干预,以减少日后喘息的发生次数。
Objective To detect the level of interleukin-33 (IL-33) in asthmatic children with asthmatic mycoplasma pneumonia (MPP), asthmatic children and non-asthmatic children, and to explore the significance and significance of IL-33 in the pathogenesis of MPP. Methods Forty-five hospitalized children diagnosed as MPP in Lishui People’s Hospital from June 2013 to August 2014 were selected as the observation subjects. According to the course of the disease in children with or without combined wheezing and (or) shortness of breath and wheezing and wheeze, the lungs were divided into wheezing group and non-wheezing group, wheezing group 15 cases, non-wheezing group 30 cases. In the same period, 25 outpatients with physical examination were collected as normal control group. The serum levels of IL-33 in MPP before and after treatment and normal control group were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The levels of IL-33 in asthmatic group and non-asthmatic group before treatment were significantly higher than those in normal control group (P <0.05). After treatment, serum IL-33 level in asthmatic group was higher than that in control group , The difference was statistically significant (P <0.05), non-wheezing serum IL-33 levels compared with the normal control group, the difference was not statistically significant (P> 0.05). Compared with the non-wheezing group, serum IL-33 levels in the two groups before treatment were significantly higher than those in the non-wheezing group (P> 0.05). After treatment, serum IL-33 level was still higher in the wheezing group than in the non-wheezing group 2 There was significant difference between groups (P <0.05). Conclusion IL-33 may be involved in the immune-inflammatory reaction of MPP. The expression of IL-33 in MPP with wheezing is longer than that of non-wheezing in MPP. MP infection with wheezing children should attach great importance to early intervention to reduce the number of wheezing in the future.