HAPTEN相关论文
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Development of a Monoclonal Antibody-Based Enzyme Immunoassay for the Pyrethroid Insecticide Deltame
With a screening hemisolid stem-cell culture, four positive hybridomas 2B12, 2C1, 2F1, and 3D4 were screened and used to......
Preparation, Identification, and Preliminary Application of Monoclonal Antibody against Pyrethroid I
Monoclonal antibodies (MAbs) against pyrethroid insecticide fenvalerate were achieved, identified, and applied in enviro......
Immunoassay Development for the Class-specific Assay for Types Ⅰ and Ⅱ Pyrethroid Insecticides in Wa
Five generic haptens of pyrethoid insecticides, which were classified as three types, were designed and synthesized: the......
Immunoassays for aflatoxin analysis have been regarded as valuable supplements to existing and rapidly developing chroma......
Synthesis and Identification of the Ciprofloxacin-Bovine Serum Albumin Conjugates for Antibody Produ
To generate the specific antibody, it was necessary to couple ciprofloxacin (CPFX) with the carrier protein.However, the......
A facile procedure was described for the hapten design of the N-methylcarbamate insecticide propoxur.Two new haptens of ......
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传统观念认为,免疫记忆是独属于适应性记忆细胞如抗原特异性的T或B淋巴细胞的。记忆性免疫细胞可以长期存活,再次遭遇相应病原体时可......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
A competitive enzyme-linked immunosorbent assay (ELISA) suitable for the determination of the urea herbicide isoproturon......
The relative molecular mass of Arbutin is small.Both fluorolabeling and radiolabeling may affect its properties and func......
Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons(PA
本文通过对荣华二采区10...
Design and efficient synthesis of novel haptens and complete antigens for the AOZ, a toxic metabolit
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
合成了保留氨基甲酸酯结构的克百威半抗原,并采用活性酯法与载体蛋白质共价连接制备突出克百威分子结构特征的合成抗原。以合成抗原......
This study reported that the hapten of the organophosphorus insecticide chlorpyrifos,O,Odiethyl-O-[3,5-dichloro-6-(2-car......
The site of attachment of protein carrier to corticosteroids has great influence on the specificity of produced antibody......
A new hapten, aldicarb oxime succinic ester (AOSE), was synthesized for immunoassay of aldicarb. It was conjugated to pr......
Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons(PA
Preparation and characterization of the hapten-protein conjugates are fundamental to developing environmental immunoassa......
目的建立抗巴比妥单克隆抗体杂交瘤细胞株,制备抗巴比妥单克隆抗体,并对其免疫学特性进行鉴定。方法将巴比妥分子环状丙二酰脲环上......
【目的】合成并鉴定斑蝥素人工抗原,为建立斑蝥素的酶联免疫分析方法提供依据。【方法】对斑蝥素进行化学修饰,引入羟基合成N-羟乙......
将伊维菌素(IVM)进行化学修饰,引入羧基活性基团,合成具有半抗原结构特征的伊维菌素半琥珀酸酯(IVM—HS);然后采用NHS法和混合酸酐法将半......
氯吡脲(Forchlorfenuron)是半抗原,只有将其与载体蛋白偶联形成完全抗原才能刺激机体产生抗体。以2-氯异烟酸为基础设计并合成了半......
通过milbemycin oxime A4、A3(MILO)肟羟基与琥珀酸酐反应,合成了半抗原MILO-5-琥珀酰半酯,采用碳化二亚胺法将其与载体蛋白BSA偶......
以4,4'-联吡啶和碘甲烷为起始原料,通氮气保护下于暗处经3步反应成功合成了百草枯半抗原N-甲基-N′-戊酸基-二吡啶二溴化物(简称PQ-h......
【目的】制备无色孔雀石绿(LMG)单克隆抗体,并分析其生物学特性,为建立LMG残留的免疫学检测方法奠定基础。【方法】通过改进的碳二亚......
【目的】合成并鉴定杠柳新苷T人工抗原,制备多克隆抗体,为进一步开展杠柳新苷类化合物作用机理的研究奠定基础。【方法】以杠柳新......
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通过保留丁酯基结构和在芳环上引入氨基取代基合成了一种邻苯二甲酸二丁酯半抗原衍生物4-氨基邻苯二甲酸二丁酯,通过1^H NMR、IR和U......
以烟草花叶病毒的赖氨酸突变体(TMV—EPMK)为半抗原载体,利用“点击化学”反应,将半抗原小分子雌三醇(E3)经由两个不同亲/疏水性的连接臂......
根据黄曲霉毒素G1(Aflatoxin G1,AFG1)的分子结构和活性位点,采用半缩醛法(SA)、环氧化物法(EP)和烯醇醚衍生物法(EED)制备G族AF人工抗原AF......
将苯巴比妥(PB)经硝化和还原两步化学修饰后,在其苯环上引入活性基团氨基,合成具有半抗原结构特征的对氨基苯巴比妥(pAPB);用重氮化法将pA......
根据敌百虫的结构特征,将其改造成新的半抗原,并分别与牛血清蛋白(BSA)和卵清蛋白(OVA)共价偶联.作为免疫原和包被原进行抗体制备及ELISA......
生物碱是自然界中广泛存在的一类天然含氮有机化合物,具有比较特殊而显著的药理活性。近年来免疫学分析技术常应用于天然药物生物碱......
【目的】获得抗川楝素的高灵敏度,高特异性多克隆抗体,建立川楝素的问接竞争酶免疫测定(IC-ELISA)方法。【方法】采用酯化反应合成川楝......
为了建立除草剂-异丙隆的酶联免疫吸附测定(ELISA)方法,以对异丙基苯基异氰酸酯和N-甲基已内酰胺为原料,经N-甲基已内酰胺水解,而......
目的:通过大黄素-BSA包被膜片的免疫原性及特异性研究,探讨半抗原-载体包被膜片人工抗原制备法的可行性。方法:先将BSA包被于PVDF膜......
以4-羧基苯甲醛,N,N-二甲基苯胺为原料合成羧基化隐性孔雀石绿(COOH-LMG),经薄层色谱、红外光谱、紫外光谱、液质联用仪鉴定该羧基化......
以2,4-二硝基氟苯为衍生剂,建立了3-氨基-2-曝唑烷酮(AOZ)的高效衍生方法,成功获得了衍生物F—AOZ,并进-步通过硝基的选择性还原合成了新......
为建立检测水产品中亚甲基蓝及其代谢物残留的酶联免疫吸附检测方法(enzyme-linked immunosorbent assay,ELISA),以亚甲基蓝代谢物......
目的:探索半抗原二硝基氟苯(DNP)修饰的恶性黑色素瘤细胞(恶黑)激活树突状细胞(DC)后,在体外诱导特异性T细胞反应的抗肿瘤效应。方法:采用DN......
合成具有阿维菌素特征结构的半抗原,采用活化酯法与载体蛋白BSA偶联制备人工抗原。通过免疫新西兰大白兔成功制备阿维菌素多克隆抗......
为检测环境和农产品中乙草胺的含量,建立间接竞争酶联免疫检测法(ELISA)。以乙草胺和3-巯基丙酸为原料合成半抗原,与牛血清蛋白偶联......
目的:制备氟甲砜霉素(FF)的最佳人工抗原并鉴定。方法:采用琥珀酸酐将FF的羟基活化后制备出半抗原氟甲砜霉素半琥珀酸(FF-HS),并经......
以除草剂二氯喹啉酸(quinclorac,Q)、氯化亚砜、氨基己酸和氨基丁酸等为起始原料,经2步化学反应分别合成了2种二氯喹啉酸半抗原:6-......
[目的]为了建立测定脲类除草剂异丙隆残留的免疫分析方法(ELISA),对制备高效价抗异丙隆多克隆抗体的方法进行了研究.[方法]以对异丙......
为了建立测定磺酰脲除草剂甲磺隆残留的酶联免疫吸附检测方法(ELISA),以邻甲酸甲酯苯磺酰胺与琥珀酸酐反应合成半抗原1-[(2-甲酸甲酯)......