从牛脑制备NSE纯品.纯化步骤依次为硫酸铵分级沉淀,DEAE—Sephacel离子交换层析,Sephacryl—s—300凝胶过滤层析和DEAE—Sephadex ......

Lignosulfonate with low polydispersity index of 1.178-1.210 was isolated by gel column chromatography of Sephacryl S-100......