论文部分内容阅读
In this paper, a sensitive and rapid method based on ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) for determination of ferulic acid, caffeic acid, vanillic acid, and paeoniflorin in rat plasma was developed.The separation of the four compounds was carried out on an AcQuity UHPLCTM BEH C18 column using a mobile phase consisting of acetonitrile and water (containing 0.1% formic acid).Electrospray ionization in positive and negative ion mode and multiple reaction monitoring was used to identify and quantitative active components.All calibration curves gave good linearity (r >0.991) over the concentration range from 4.24-265 ng / mL to 11.5-2875 ng / mL for all components.The precision of the in vivo study was evaluated by intra-and inter-day assays and the percentages of RSD were all within 10.6%.The recovery ranged from 61.5 to 75.4%.The method was successfully applied to pharmacokinetic study of all three aromatic acids and one monoterpene in rat plasma.Furthermore, we comPared the pharmacokinetics profile of four compounds in normal and primary dysmenorrhea rats plasma following oral administration of Shaofu Zhuyu decoction (SFZYD) and its ethanol supematant extract (SFE).