The targeted mutations of interesting genes are essential for the functional genome analysis.TILLING (Targeting Induced Local Lesions in Genomes), a powerful reverse genetics tool, can be used to screen a series of mutants in a mutagenized population.In the last few years, we had set up TILLING platforms for tobacco and rice by using EMS-mutagenized populations with 1 051 and 5 200 M2 lines, respectively.