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This study was aimed at determining whether the epidemiology of PRRSV involved the hybrid wild boars.We conducted a serologic investigation of porcine reproductive and respiratory syndrome virus (PRRSV) in hybrid wild boar herds in China during 2008-2009.613 blood samples from Shandong Province were obtained from hybrid wild boars that had not been vaccinated against PRRSV.167 (27.2%) of 613 samples were seropositive for PRRSV,indicating that the prevalence of PRRSV differed between domestic pigs and hybrid wild boars in this area,and 2 PRRSV isolates (TAYZ and ZCYZ) were plaque-purified once in MARC-145 cells and amplified for sequencing and infection of other pigs.The result showed TAYZ and ZCYZ had higher nucleotide homologies in ORF5 with highly pathogenic PRRSV (prototype virus JXA1).