With rapid development of graphene oxide(GO)in biomedical technology increasing importance has been attracted to their biosafety in recent years[1].Herein,we investigated the acute activation of inflammatory cascades upon exposure to GO as well as detailing the mechanisms.Firstly,IL-6-Fluc reporter gene was targeted to mouse liver by hydrodynamic gene delivery technology[2],which was selected as ideal index for acute inflammation of the liver.The reporter model mouse was able to visualize IL-6 activation at the transcriptional stage[3],with higher sensitivity than the ELISA method.