酿酒酵母菊芋乙醇代谢工程

来源 :2015年中国微生物学会学术年会 | 被引量 : 0次 | 上传用户:shena011
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菊糖是植物的第二大储存多糖,仅次于淀粉.菊糖由果糖基通过β-2,1-糖苷键连接,末端通常结合一分子葡萄糖,菊糖的聚合度在2-60之间.菊芋由于块茎富含菊糖、能在非耕土地种植,被认为是菊糖的可持续来源和潜在的生物能源植物.利用酿酒酵母转化菊糖生产燃料乙醇,要求菌种能够直接发酵菊糖,而野生型酿酒酵母没有菊糖酶基因,不能高效转化菊糖.本研究应用组合代谢工程策略,通过在酿酒酵母宿主中表达外源外切菊糖酶和内切菊糖酶、优化酶的表达量和分泌水平、调节宿主蛋白降解系统、以及改变宿主倍性等,系统地改造了酿酒酵母菌株,获得了高效的酿酒酵母菊芋乙醇整合生物加工菌种.
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