Starch is the most important food energy source in cereals.Many of the known enzymes involved in starch biosynthesis are partially or entirely granule-associated in the endosperm.So the proteome of rice starch granule is important for us to further understand the mechanisms underlying starch biosynthesis and starch granules packaging in rice amyloplast.To look for new candidate proteins which may be involved in starch biosynthesis in rice endosperm,we developed a protocol to purified starch granules from mature rice endosperm and verified the quality of the starch granules by microscopy observations,I2 staining,and Western blot analyses.