Objective To determine the full-length genome sequences of hepatitis delta virus (HDV) from the sera of a HDV RNA-positive subjects using reverse transcription polymerase chain reaction (RT-PCR).Metho
根据NCBI提交的刺参甘露糖结合凝集素(Mannan-binding lectins,MBLs)序列(AY625513)设计引物,抽取刺参体腔液进行总RNA提取,采用RT-PCR技术,获得498bp目的片段。通过克隆、测序确定得到刺参MBLs序列。将得到序列进行核苷酸和氨基酸序列比对,首次鉴定了大连刺参MBLs序列,并为我国不同区域刺参的进一步分类提供了科学的分子水平依据。
It has been suggested that ezrin activation plays a key role in the regulation of cancer metastasis.In this study,we immunohistochemically investigated the expression patterns of total ezrin and its t
目的：通过转染hTERT基因构建强直性脊柱炎成纤维细胞永生化细胞系并观察期转化特征。方法：取强直患者韧带组织通过胶原酶消化法分离培养原代成纤维细胞。细胞稳定传代后转染PCI-NEO-hTERT质粒，经G418筛选得到阳性克隆，连续传达培养。应用PCR-ELISA法检测细胞端粒酶活性。采用细胞形态学观察、染色体核型分析、免疫荧光分析转染细胞是否具有转化特征。结果：转染后获得阳性克隆扩大培养和生长良好
Biophoton emission is the spontaneous emission of ultraweak light emanating from all living systems,including man.It is an intrinsic property of living things and contains a plenty of biological infor
Biophotonics is an interdisciplinary of optical technology and life sciences,which can study cell function and structure at the molecular level,and contain a lot of biological information.People can d
强直性脊柱炎(Ankylosing Spondylitis,AS)是一主要侵犯骶髂关节、脊椎关节与附近的肌腱、韧带等软组织,炎症后继发纤维化与异位骨化,使脊椎逐渐失去柔软度,严重时会发展到像“竹节”一样无法弯曲或伸展.在目前已经发现的与强直性脊柱炎有关的多个易感基因中,与AS异位骨化关系最密切的是BMP基因,其他AS易感基因如ARTS1,ILR23可能也与骨化有关,但还需进一步研究,AS骨化易感基
Peptidylarginine deiminase type 4 (PADI4) converts arginine residues into citrulline.The current study focused on the expression of PADI4 in various subtypes of ovary cancers,and this study investigat
研究背景：儿茶酚胺类神经递质(去甲肾上腺素、肾上腺素和多巴胺)发挥重要的生理作用。其在组织液和体液中的水平与许多的生理和疾病状态密切相关。但这类神经递质在组织基质中的含量很低，且易分解。故发展有效的稳定和富集单胺的方法，对检测显得尤为重要。目的：本研究合成二巯基丁二酸修饰的磁性纳米颗粒(DMSA@MNPS)，其通过静电吸附作用形成磁性聚集体，用于儿茶酚胺类神经递质的富集(原理如图1示)，并进行洗脱