【摘 要】
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Aim to observe the effects of electromagnetic pulse on mRNA expression of Fas and FasL in murine splenocytes.Methods Forty male Balb/c mice with body mass of (18±2)g were randomly designated non-irrad
【机 构】
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Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University
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Aim to observe the effects of electromagnetic pulse on mRNA expression of Fas and FasL in murine splenocytes.Methods Forty male Balb/c mice with body mass of (18±2)g were randomly designated non-irradiation group (8 mice)and whole-body irradiation group (32 mice).Mice received irradiation of electromagnetic pulse for two days.25 pulses one day with about 2 minutes interval.Two non-irradiated mice and 8 irradiated mice were sacrificed and their spleens were removed 2 hours, 4hours, 8hours and 12 hours after irradiation, respectively, RNA dot blotting assay was employed to examine the expression levels of Fas and FasL gene in murine splenocytes.Positive signals were scanned for gray density and RNA expressing index were calculated.Results Compared with control group, the RNA expressing index of Fas and FasL in murine splenocytes increased markedly 2 hours after EMP irradiation.0.97±0.25 vs 0.59±0.26 for Fas and 0.96±0.21 vs 0.56±0.23 for FasL.However, the RNA expressing index of Fas and FasL decreased slightly 2 hours later (0.75±0.24 and 0.69±0.22, respectively), then increased to the highest levels (p<0.001 vs cotrol group) 8 hours after irradiation (1.22±0.22 and 1.32±0.50 respectively),and finally went down at 12 hours to the normal level.Conclusions EMP irradiation can cause an undulatory increasing expression of Fas and FasL in muine splenocytes.
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