【摘 要】
：
目的 旨在通过对比正常形态精子和典型畸形精子的拉曼峰谱,深入了解精子有机成分的组成,并借助拉曼光谱建立一种非侵入性精子形态的分析检测方法.方法 以6例捐精者正常形态精子为实验组(其中包括一例已被证实使女性受孕),以3例典型畸形的精子样本为对照组,其中一例为圆头精子症,精子形态学分析显示为100％圆头精子;第二例为严重畸形精子症,精子头自颈部与尾部分离,形态学分析显示98％的精子形态出现异常,第三例
【机 构】
：
上海交通大学医学院附属仁济医院泌尿科 上海市人类精子库 上海市辅助生殖与优生重点实验室 上海交通大
【出 处】
：
中华医学会第七次全国生殖医学学术会议
论文部分内容阅读
目的 旨在通过对比正常形态精子和典型畸形精子的拉曼峰谱,深入了解精子有机成分的组成,并借助拉曼光谱建立一种非侵入性精子形态的分析检测方法.方法 以6例捐精者正常形态精子为实验组(其中包括一例已被证实使女性受孕),以3例典型畸形的精子样本为对照组,其中一例为圆头精子症,精子形态学分析显示为100％圆头精子;第二例为严重畸形精子症,精子头自颈部与尾部分离,形态学分析显示98％的精子形态出现异常,第三例为原发性不育(不育年限达12年)精子标本,精子标本被IVF中心确诊为完全受精失败.根据传统精子形态分析方法,按精子的顶体、颈部、尾部大小划分5个扫描区域,每条精子共要扫描23个点,从9份精子样本中,每例选取了5条精子,每条精子经2名技术员重复检测,共做了45条精子共90次的拉曼光谱分析.
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