Preparation and in vitro evaluation of aptamer conjugated lipid-polymer hybrid nanoparticles for MUC

来源 :2014年中国药物制剂大会——中国药学会药剂专业委员会年会、第六届亚洲阿登制药技术研讨会暨国际控释协会中国分会年会 | 被引量 : 0次 | 上传用户:myloft1d
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Objective: In this research, a novel vinorelbine-loaded aptamer (Apt) conjugated lipid-polymer hybrid nanoparticles (Apt-NP/VRL) were constructed for specific targeting MUC 1 protein over expressed tumor cells.Methods: Apt-NP/VRL were prepared by self-assembling method using lecithin, DSPE-PEG2ooo-COOH, and poly (lactic-co-glycolic acid) (PLGA) as carrier material.The formulation was optimized by orthogonal design.The aptamer was conjugated to DSPE-PEG2000-COOH through EDC and NHS coupling (Figure 1).Though changing the ratio of DSPE-PEG200o-Apt, NPs with different aptamer densities were synthesized and characterized.Courarin-6 labeled nanoparticles (Apt-NP/Cou-6) were prepared to evaluate the cellular uptake efficiency.The anti-tumor activity of Apt-NP/VRL to MCF-7 cell and HpG2 cell was evaluated.
其他文献
Attention-deficit/hyperactivity disorder (ADHD) is an impairing neurobehavioral condition, clinically characterized by inattention, hyperactivity, and impulsivity.Estimated to have a worldwide pooled
会议
This work determined the mechanisms of enhancing the oral bioavailability of syringaresinol (SG).The in situ perfusion method was used in rats to study the absorption mechanisms of SG alone or combine
Objective Folate receptors (FRs), over-expressed on the surface of a variety of tumor cells, are potential targets for tumor targeting therapy.This is to continue our earlier work on the folate conjug
A major bottleneck in cancer chemotherapy therapy is to achieve specific drug accumulation at tumor site.Nanotechnology-based therapeutic products have been approved to target delivery drug for clinic
Glioma, the most aggressive and common intracranialtumor, is considered as the major cause of death from central nervous systems (CNS).Although various methods have been explored to conquer it, includ
会议
Especially in recent years, photothermal therapy (PTT) mediated by near-infrared (NIR)-resonant nanomaterials has aroused widespread interest as a minimally invasive treatment methodology.An especiall
会议
Objective: The objective of this study was to investigate the feasibility of MD as a tool to determine the skin concentration of mometason furoate (MF), a lipophilic, highly protein-bound compound.
会议
Mucosal immune response builds a first barrier for the entry of the vast majority of pathogens which are transmitted through mucosa, such as influenza, Ebola, EV71 virus etc.Moreover, mucosal vaccinat
Objective: In the past few years, aptamer physisorbed on graphene oxide (GO) has emerged as a useful sensing platform In a typical assay, a fluorescently labeled aptamer probe is adsorbed by GO and th
Objective: To achieve the synchronous skin permeation of indapamide (IND) and bisoprolol (BSP), two drugs with totally different skin permeability, the technique of ion-pair complexation and chemical
会议