【摘 要】
：
Aim: To research the mechanisms of the interaction between the two important channels in repolarization reserve.Methods: According to the transient transfection of different genes, HEK293 cells were d
【机 构】
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Institute of Geriatric Cardiology, the General Hospital of PLA, Beijing, China
【出 处】
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The 4th International Ion Channel Conference(第四届国际离子通道会议)
论文部分内容阅读
Aim: To research the mechanisms of the interaction between the two important channels in repolarization reserve.Methods: According to the transient transfection of different genes, HEK293 cells were divided into seven groups: KCNQ1 (Q1), and WT-KCNH2 co-transfection (Q 1 +WT), KCNQ 1 and T618I-KCNH2 (gain of function mutation, Q 1 +T618I), and V535M-KCNH2 (loss of function mutation, Q1 +V535M) group.The total RNA and protein were extracted, detected by RT-qPCR and Western blot.KCNQ1 current changes induced by different functional KCNH2 were recorded by whole-cell patch clamp technique.
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