【摘 要】
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We have developed a technology platform which has been referred to as multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL).MDMS-SL is comp
【机 构】
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DiabetesandObesityResearchCenter,Sanford-BurnhamMedicalResearchInstitute,Orlando,FL32832,USA
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
We have developed a technology platform which has been referred to as multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL).MDMS-SL is comprised of the components of multiplexed sample preparations, intrasource separation, MDMS identification, two-step quantification, and bioinformatic data analysis.This technology platform represents one of the most well-developed, powerful, and high throughput approaches in the lipidomics field.The technology enables us to identify and quantify over 95% of total cellular lipid mass, up to thirty lipid classes, and thousands of individual lipid molecular species in an unbiased manner from a minimal amount of source materials.This technology has been successfully applied to numerous biological and biomedical studies.
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