论文部分内容阅读
目的:探讨ABCB1基因单核苷酸多态位点rs2235048在中国汉族人群中的分布及其与氯吡格雷抵抗(Clopidogrelresistance,CR)发生的相关关系。方法:采用光学比浊法测定20μmol/L ADP诱导的残余血小板聚集率(Residual plateletagglutination,RPA)。当RPA≥70%时,即为CR。所有入选患者分为CR组和氯吡格雷非抵抗组(Non-clopidogrel resistance,NCR)。采用焦磷酸测序法测定ABCB1基因rs2235048单核苷酸多态位点在CR组和NCR组的基因型及等位基因分布频率。结果:ABCB1基因rs2235048多态在CR组和NCR组基因型分布频率符合Hardy-Weinberg平衡。在CR组和NCR组中,ABCB1基因rs2235048多态的基因型频率分布无统计学差异(P=0.527,X2=1.281);T、C等位基因频率在两组间分布频率也没有统计学差异(P=0.740,OR=0.958,95%CI=0.742～1.236)。结论:对PCI术后服用氯吡格雷的冠心病患者进行分析发现,ABCB1基因单核苷酸多态位点rs2235048与冠心病患者CR的发生无相关关系。
Objective: To investigate the distribution of rs2235048 ABCB1 SNP in Chinese Han population and its relationship with Clopidogrel resistance (CR). Methods: Residual platelet aggregation (RPA) induced by 20 μmol / L ADP was measured by optical turbidimetry. When RPA ≥ 70%, is CR. All patients were divided into CR group and non-clopidogrel resistance (NCR). The genotypes and allele frequencies of rs2235048 SNP in ABCB1 gene in CR group and NCR group were determined by pyrosequencing. Results: The genotype frequency of ABCB1 rs2235048 polymorphism in CR and NCR groups was in accordance with Hardy-Weinberg equilibrium. In CR group and NCR group, genotype frequency of rs2235048 polymorphism of ABCB1 gene had no statistical difference (P = 0.527, X2 = 1.281). The frequency of T and C allele had no statistical difference between the two groups (P = 0.740, OR = 0.958, 95% CI = 0.742 ~ 1.236). Conclusion: The analysis of clopidogrel in patients with coronary heart disease after PCI found that ABCB1 gene SNP rs2235048 had no correlation with the occurrence of CR in CHD patients.