LSPR-based sensors are promise candidate as high-throughput screening(HTS)platform[1] for biochemical sensing and drug screening.The transducer spots can be minimized to a small number of individual sensing elements with an in-plane width of less than ~100 nm[2].Previously,we had demonstrated that plasmonic gold nanomushroom array(GNMA)substrate offered a state-of-the-art system for label-free optical biosensing with refractometric figures of merit approaching the theoretical limit[3].In this paper,a low-cost GNMA microarray chip consists of integrated cells was prepared.The as-obtained GNMA were applied to convenient normal transmission measurement and showed excellent refractive index sensitivity and FOM value.Such a high sensitivity enabled label-free detection of glucose and cholesterol,with limits of detection down to 67.9 mg/dL and 92.5 mg/dL,respectively.Notably,four medical analytes were simultaneously detected on a microarray chip integrated with GNMA sensing component,suggesting that the GNMA sensing substrate is a promising candidate for high-throughput monitoring of multiple analytes.