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The type Ⅱ clustered, regularly interspaced, short palindromic repeat (CRISPR) / CRISPR-associated protein 9 (Cas9) system is a novel molecular tool of site-specific genome modification.Very recently, the CRISPR-Cas9 system was reported to be successfully performed by transient or biolistic-mediated stable transformation in Arabidopsis, Nicotiana benthamiana and rice.