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National and regional pharmacopeial compendia provide analytical methods for the measurement of important components of drug substances and drug products.These accepted methods have been in use for an extended period and often reflect their original development on the chromatographic instrumentation and column chemistry in common use several years ago.It is desirable to transfer these methods to currently offered instruments and separation chemistries that offer the benefits of recent chromatographic innovations.This method migration has become particularly important with the widening acceptance of UltraPerfomance Liquid Chromatography (UPLC) techniques.This class of separation science is based on using columns packed with sub-2 m particles on instruments optimized to preserve the reduced band-broadening of such particles.The UPLC separations are characterized by improved resolution and better sensitivity, typically in a shorter run time with higher throughput.