【摘 要】
：
Laser tweezers Raman spectroscopy (LTRS) was used to investigate the Raman spectra of the mitochondrias of yeast cells in vitro after induced with acetic acid.The results showed that the spectral peak
【机 构】
：
Biophysics Laboratory, Guangxi Academy of Sciences,nanning, china
【出 处】
：
The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
Laser tweezers Raman spectroscopy (LTRS) was used to investigate the Raman spectra of the mitochondrias of yeast cells in vitro after induced with acetic acid.The results showed that the spectral peaks of the nucleic acid (1081,1301cm-1), the peaks of proteins (872, 1604, 1445, 1657cm-1), the peaks of lipids (1301, 1604, 1445, 1657cm-1),the peaks of Cyt c (750,, 1125cm-1), the peaks of mitochondrial respiration (1604cm-1) were decreased significantly as a function of the duration of the cetic acid stress, which was similar with the physiological and biochemical indexes of respiration rate, p/ o ration,cyt c content, implied that acetic acid could penetrate into the cell interior and directly impacted the mitochondria possibly, resulted in the release of inclusions from the mitochondrias, subsequently, caused the apoptosis of yeast cells which relies on the mitochondrial pathways.LTRS is a non-invasive tool for mitochondria analysis at a single mitochondrial level.
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