【摘 要】
：
RECK is a membrane-anchored glycoprotein which may negatively regulate matrix metalloproteinase (MMP) activity to suppress tumor invasion and metastasis.In this study, recombinant proteins correspondi
【机 构】
：
Institute of Biomedical Sciences National Sun Yat-Sen University Taiwan
【出 处】
：
BIT Life Sciences 1st Annual World Cancer Congess-2008(2008中
论文部分内容阅读
RECK is a membrane-anchored glycoprotein which may negatively regulate matrix metalloproteinase (MMP) activity to suppress tumor invasion and metastasis.In this study, recombinant proteins corresponding to the residues 285-368 (named as CKM which contained cysteine knot motif), 605-799 (named as K123 which contained three Kazal motifs), 676-799 (named as K23 which contained the last two Kazal motifs) and full-length RECK were produced and their anti-cancer effects were tested.Full-length RECK and K23 but not K123 and CKM inhibited MMP9 secretion and activity.In addition, RECK and K23 inhibited invasion but not migration of metastatic lung cancer cells in vitro.Protein binding and kinetic study indicated that K23 physically interacted with MMP-9 and inhibited its activity by a non-competitive manner.Moreover, K23 reduced metastatic tumor growth in lungs of nude mice.Taken together, our results suggest that the K23 motifs of RECK protein can inhibit MMP-9 secretion and activity and attenuate metastasis of lung cancer cells.
其他文献
Focal Adhesion Kinase (FAK) is expressed and activated at higher levels in malignant cells than in normal cells.Previously we reported that FAK knockdown in B-ALL cells causes impaired chemotactic and
Purpose: The primary aim of this study was to assess the efficacy of the Short-Term Life Review (STLR) on the spiritual well-being, as well as anxiety, depression, suffering, and happiness of terminal
Colorectal cancer is the second leading cause of cancer related death in the Western World.Czech Republic is in the first place in worldwide epidemiological statistics following incidence and mortalit
KMUP-1is aα 1A/α 1D-adrenoceptor antagonist.Previously, we have demonstrated that KMUP-1 can regulate smooth muscle tone and inhibit epithelial cell proliferation in the prostate.Prostate cancer usual
Beclin 1 was the first autophagic gene identified in mammarlian cells with roles in mediating autophagy and apoptosis.Earlier studies showed that beclin 1 expression was down-regulated with mono-allel
Dendritic cells (DCs) display an extraordinary capacity to induce, sustain and regulate T cell responses providing the opportunity of DC-based cancer vaccination strategies.Additional findings indicat
Hepatocellular carcinoma (HCC) mostly develops in the liver under chronic inflammation.Oxygen radicals produced at the site of inflammation attack membrane bound and circulating lipids forming a numbe
In liver, TGF-beta limits the regenerative tissue growth after injury by inhibiting DNA synthesis and inducing apoptosis.Derangement of TGF-beta signaling is associated with an increased incidence of
The significant advances in microarray and proteomics analyses have resulted in an exponential increase in potential new targets and have promised to shed light on the identification of disease marker
The Drosophila dachshund (dac) gene is a helix-turn-helix nuclear protein that governs cell fate determination.Dac is essential for the development of the drosophila eye and limb.The dac gene forms pa