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PYR1/PYL/RCAR proteins (PYLs) are confirmed abscisic acid (ABA) receptors,which inhibit protein phosphatase 2C (PP2C) upon binding to ABA.Arabidopsis has at least 14 PYLs.Through systematic biochemical characterization of PYL, we identified a subclass of PYLs, represented by PYL10, that inhibited the phosphatase activity of PP2C in the absence of any ligand.