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It has been established that microRNAs(miRNAs),a family of important small non-coding RNA molecule,play important roles in embryonic development of the zebrafish,but more detailed mechanisms are still unclear.Here we studied the roles of miR-202,a microRNA highly expressed in gonad,in the regulation of embryonic development of the zebrafish.First we found that microinjection of zebrafish embryos using LNA-modified antisense oligonucleotide against miR-202 resulted in blockage of embryonic development at 6h after fertilization.Further study showed that the expression level of miR-202 was very low right after fertilization and then increased remarkably and reached peak at 4h after fertilization during the embryonic development.Microarrary profiling of gene expression showed that expression of more than 1000 genes changed after injection of miR-202-antisense oligonucleotide and 37 significantly dysregulated genes were predicted as direct targets of miR-202,those genes are involved in the regulation of transcription,cell cycle,apoptosis,sex determination,etc.The present data indicate that miR-202 play essential roles in the early embryonic development in zebrafish and loss of miR-202 function blocked the embryonic development at 6h after fertilization,multiple signaling pathways may be involved in this process.