Floral Zygomorphism is a classical model in the study the flower shape patterning.As typical flower morphology evolves, it manifests two kinds of asymmetries in floral and organ planes: dorsoventral (DV) and organ internal (IN) asymmetries.Previous studies have demonstrated that several CYC-like TCP transcription factors were recruited independently in the control of zygomorphic flower development in high plants.However, the biochemical basis for CYC-like TCP protein and the related signaling during zygomorphic flower development is still largely unknown.Bioinformatics and genetic analysis showed that multiple modifications were predicted in the protein and many modification site mutations have resulted in strong phenotypes, indicating that these modifications have a crucial role in their functions.The sequence analysis showed that there was a conserved TCP domain and R domain respectively in the N terminal and C terminal of these proteins.Protein truncation analysis suggested that the N-terminal (TCP domain included) is critical for whole protein function while the C-terminal perhaps plays a regulatory role.