【摘 要】
：
Soil waterlogging is one of the major abiotic stresses affecting maize grain yields.To understand the molecular mechanisms underlying waterlogging tolerance in maize,the iTRAQ LC-MS/MS technique was e
【机 构】
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National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Ch
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Soil waterlogging is one of the major abiotic stresses affecting maize grain yields.To understand the molecular mechanisms underlying waterlogging tolerance in maize,the iTRAQ LC-MS/MS technique was employed to map the proteomes of seedling root cells of the A3237 (tolerant inbred) and A3239 (sensitive inbred) lines under control and waterlogging conditions.Among the 3318 proteins identified,211 were differentially abundant proteins (DAPs),of which 81 were specific to A3237 and 57were specific to A3239.These DAPs were categorized into 11 groups that were closely related to the plant stress response,including metabolism,energy,transport and disease/defense.In the waterlogged A3237 root cells,NADP-malic enzyme,glutamate decarboxylase,coproporphyrinogen Ⅲ oxidase,glutathione S-transferase,glutathione dehydrogenase and xyloglucan endotransglycosylase 6 were specifically accumulated to manage energy consumption,maintain pH levels and minimize oxidative damage.
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