【摘 要】
：
Chronic renal failure (CRF) is defined as a progressive and irreversible loss of renal function and associated with inflammation and oxidative stress.Salvia miltiorrhiza (SM) is an important Chinese h
【机 构】
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Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National a
【出 处】
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2016首届中国中药资源大会暨CSNR中药及天然药物资源研究专业委员会第十二届学术年会
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Chronic renal failure (CRF) is defined as a progressive and irreversible loss of renal function and associated with inflammation and oxidative stress.Salvia miltiorrhiza (SM) is an important Chinese herb used in traditional Chinese medicine with notable effects on CRF.Here, we showed that the ethanol extract (SMEE) and water extract (SMWE) of SM significantly inhibited the elevation of serum creatinine (Scr), blood urea nitrogen (BUN), urine protein (UP) and indoxyl sulfate (ISF) in adenine-induced CRF rats, especially SMEE.Moreover, SM extracts obviously improved the symptoms of glomerular and tubular atrophy, focal calcium deposits, interstitial fibrosis, interstitial inflammation,and renal tissues.Fifty-nine metabolites (thirteen in plasma, twenty-seven in urine and nineteen in kidney tissue) were up-regulated or down-regulated and contributed to CRF progress.After treatment of SM extracts, the altered metabolites were restored back to normal level.These potential biomarkers underpinning the metabolic pathways are including phenylalanine metabolism, pyrimidine metabolism,purine metabolism and tryptophan metabolism.Furthermore, SM extracts prevent epithelial-mesenchymal transition (EMT) of Human renal tubular epithelial (HK-2) cell by inhibiting NADPH oxidase/ROS signaling pathway and tanshinone ⅡA (DⅡA), salvianolic acid B (SAB) were the main bioactive compounds, providing a powerful supports to develop a new therapeutic agent for CRF.
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