【摘 要】
：
Objective To investigate how does the addition of plant oil in the diet influence the ω-6 /ω-3 PUFAs ratio in the heart and the effect of those on the expression of cytochrome P450 2J (CYP2J) and the
【机 构】
：
Department for VIP,Capital Medical University affiliated Beijing Anzhen hospital,Beijing Institute o
论文部分内容阅读
Objective To investigate how does the addition of plant oil in the diet influence the ω-6 /ω-3 PUFAs ratio in the heart and the effect of those on the expression of cytochrome P450 2J (CYP2J) and the myocardial injury due to ischemia/reperfusion.Methods 80 C57BL/6 mice, randomly divided into ordinary diet group and plant oil group.One month later, myocardial ischemia/reperfusion model was built in these mice, 2,3,5 triphenyltetrazolium chloride (TTC) staining was used to measure infarction size.real time reverse transcription-polymerase chain reaction(RT-PCR) and western blot was used respectively to detect the mRNA and protein expression of CYP2J.ω-6/ω-3 PUFAs and EET were tested by high-performance gas chromatography.Results n-6/n-3 PUFA ratio in the heart decreased in the plant oil group than in the ordinary diet group, after ischemia/reperfusion, the expression of CYP2J and the amount of EET in the area at risk was higher in the plant oil group, Infarction size of plant oil group were diminished.Conclusion Properly increase plant oil intake lower the n-6/n-3 PUFA ratio, improve the expression of CYP2J and the ischemia/reperfusion injury.
其他文献
Objective The ectopic foci originating from superior vena cava (SVC) may act as an triggers in the occurrence and perpetuation of atrial tachyarrhythmia.The common complications of isolation of SVC we
背景 阵发性房颤终止后出现的症状性长间歇往往需要植入永久性心脏起搏器,随着导管消融技术的不断成熟,该技术已经成为症状明显、药物治疗无效阵发性房颤的一线治疗方法,本研究旨在评估导管消融对阵发性房颤相关快-慢综合症的疗效.方法 本研究连续入选了2010年1月至2012年9月在北京安贞医院房颤中心接受导管消融的50例阵发性房颤合并房颤终止后症状性长间歇(阵发性房颤相关快-慢综合症)的患者,这些患者作为导
研究背景 既往大量流行病学研究显示,高密度脂蛋白胆固醇(HDL-C)水平低是心血管病(CVD)事件发生的危险因素.然而近期的几项大型随机对照临床试验结果表明,升高HDL-C并未有效降低CVD事件的发生率.HDL-C可能并不能完全反映高密度脂蛋白(HDL)的心血管保护作用.HDL作为运输胆固醇的载体,它与CVD之间的关联正在被越来越多的研究者关注.研究目的 在我国中老年人群中分析高密度脂蛋白(HDL
Objective To investigate the effect of structural design of artificial disc on the biomechanical changes after disc replacement.Methods Based on clinical images, we built a three dimensional finite el
Objective To explore the emotional disorders (anxiety and depression) and the hospital anxiety and depression scale (HADS) , the Zungself evaluation scale (SAS/SDS), Hamilton anxiety and depression sc
Objective To observe the effect of Yinxingguttate on plasma intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), nitric oxide, malondialdehyde in patients with stabl
Objective To study the correlation between serum high sensitivity C-reactive protein (hs-CRP) and carotid atherosclerosis.Methods 161 Volunteers from a community in ~ city aged from 25 to 90 were coll
Objective To build a composite score based on the Global Registry of Acute Coronary Events (GRACE) score and N-terminal pro-brain natriuretic peptide (NT-proBNP) concentrations to predict outcome in p
Background Statins and ezetimibe have been reported to change the balance of cholesterol metabolism, but few studies have been performed on Chinese patients.The aim of this study was to evaluate chang