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The silkworm, Bombyx mori, is a model insect of Lepidoptera, and the innate immune defense mechanism is becoming a hot research topic.Malpighian tubules (MTs) of insect is an autonomous immunologic tissue, which can start an effective immune response entirely independent of the fat body.Recent studies show that miRNAs play an important role in anti-viral infection.In this paper, we investigated on MTs of silkworm response to BmNPV virus infection at miRNA levels.