【摘 要】
：
镉(Cd)是一种重金属,其毒性对动物的健康构成危险。而硒(Se)是一种重要的营养微量元素。有相关报道指出,镉与硒具有相互的颉颃作用,而二者在禽类中是否存在颉颃作用还未见报道。该研究的目的是通过检测镉和硒对鸡脾脏淋巴细胞中4个内质网相关硒蛋白mRNA的变化,探讨镉毒性和硒保护作用的可能机制。在体外培养鸡脾脏淋巴细胞的培养液中分别添加硒10-7 mol/L、镉10-6 mol/L及硒镉混合物10-7
【机 构】
：
东北农业大学动物医学学院临床兽医内科教研室,黑龙江哈尔滨150030
【出 处】
：
中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
镉(Cd)是一种重金属,其毒性对动物的健康构成危险。而硒(Se)是一种重要的营养微量元素。有相关报道指出,镉与硒具有相互的颉颃作用,而二者在禽类中是否存在颉颃作用还未见报道。该研究的目的是通过检测镉和硒对鸡脾脏淋巴细胞中4个内质网相关硒蛋白mRNA的变化,探讨镉毒性和硒保护作用的可能机制。在体外培养鸡脾脏淋巴细胞的培养液中分别添加硒10-7 mol/L、镉10-6 mol/L及硒镉混合物10-7 mol/L和10-6 mol/L,分别在培养的12、24、36和48 h收集细胞用于后续试验的检测。采用实时定量PCR法检测4个内质网硒蛋白,即硒蛋白K、N、S、T的mRNA表达量,结果表明,镉能够降低体外培养鸡淋巴细胞硒蛋白的表达量,而硒能够显著提高硒蛋白mRNA的表达量。通过对硒蛋白之间的相关性分析,表明硒对镉诱导的淋巴细胞损伤具有保护作用。
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