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Aptamers are oligonucleotide that bind to a specific target molecule[1].Nucleic acid aptamers have been usually engineered through systematic evolution of ligands by exponential enrichment (SELEX)[2].The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is participated in variety of tumors development[3].BC15 is a DNA aptamer that could specifically recognize the hnRNP A1[4,5].