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The interaction between 3-spiro-2-pyrrolidine-3-spiro-3"-piperidine-2,3"-dione(PPD) and bovine serum albumin (BSA) in aqueous solution has been studied using fluorescence and UV–vis spectroscopy in this work.Fluorescence emission data revealed that BSA (1.00×10-5 mol·L-1) fluorescence was statically quenched by PPD at various concentrations,which implies that PPD–BSA complex has been formed.The binding constant (KA * Corresponding author.Tel.: +86-731-58290187; Fax: +86-731-58290509.) was calculate,the individual values had been compared as in the following order: compound 4 < compound 3 < compound 2 < compound 1 at different temperatures,and had a discussion on different results when the four aimed compounds interacted with BSA.The number of binding sites (n) and the specific binding sites of the PPD with BSA were determined.Energy–transfer efficiency parameters were analyzed,moreover,the distance (r) between BSA and PPD had been compared to illustrate the interaction between different PPD and BSA.According to van’t Hoff equation,the thermodynamic parameters,ΔG,ΔH and ΔS,were obtained,ΔH>0,ΔS>0,showing that the involvement of hydrophobic force in these interactions; ΔG<0,indicated the binding of PPD with BSA is spontaneous.The synchronous fluorescence of BSA was affected by compounds which had slight blue or red shift which showed that PPD had caused some structural changes to BSA.