During development of mammalian animal,dopamine neurons in midbrain form mesostriatal,mesocortical and mesolimbic projections,which are relative with motor control,mood and reward respectively.Among these three projections,mesolimbic DA projection is the neurobiological basis for drug dependence.Previous studies have showed that drug addiction shares the common molecular which regulates mesolimbic projection with rewarding.However,little is known about the molecular mechanism of mesolimbic projection.In our study,these mesolimbic DA subtype neurons were isolated by neuronal labeling and fluorescence activated cell sorting,and the specific genes and microRNAs were screened with microarry analysis of whole gene expression and microRNA expression.As the specific genes and relative pathways,miR-212/132 and Mecp2 pathway,mir-133b and extracellular signal regulated kinase pathway were selected to analyze their functions.The data showed that miR-212/132 and Mecp2 were closely related with methylamphetamine induced behavioral sensitization in mouse.Mir-133b and extracellular signal regulated kinase could also regulate methylamphetamine induced neurotoxicity.The current results will be helpful for understanding the molecular mechanisms of mesolimbic projection and potentially lead to a possible approach for intervening methylamphetamine dependence.