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An antagonistic Bacillus amyloliquefaciens CGMCC6978, originally isolated from the permafrost soil samples collected from the Mohe, Heilongjiang province, China.It showed a strong biocontrol activity against a large number of phytopathogenic fungi and bacteria.In this study, the traits of biocontrol activities of B.amyloliquefaciens CGMCC6978 were characterized and effects of the strain in controlling grey mold on tomato leaves and fruits were examined by treating them with the fermentation broth of CGMCC6978, and moreover, the detection of lipopeptide synthesis related genes by using specific primers were carried out.