【摘 要】
：
目的：采用免疫磁珠(MACS)联合定量PCR法检测胰腺癌患者外周血循环肿瘤细胞(CTCs),分析冷冻消融术前后CTCs变化及其与患者预后的关联性.方法：收集2011年11月至2013年6月暨南大学医学院附属复大肿瘤医院收治的20例行冷冻消融术的胰腺癌患者,使用MACS联合定量PCR法检测患者术前及术后10d外周血CTCs.治疗后随访2年,记录患者的预后,分析CTCs对患者预后的预测价值.结果：20
【出 处】
：
2016第五届国际(广州)癌症治疗论坛暨国际干细胞再生医学学会成立大会
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目的：采用免疫磁珠(MACS)联合定量PCR法检测胰腺癌患者外周血循环肿瘤细胞(CTCs),分析冷冻消融术前后CTCs变化及其与患者预后的关联性.
方法：收集2011年11月至2013年6月暨南大学医学院附属复大肿瘤医院收治的20例行冷冻消融术的胰腺癌患者,使用MACS联合定量PCR法检测患者术前及术后10d外周血CTCs.治疗后随访2年,记录患者的预后,分析CTCs对患者预后的预测价值.
结果：20例患者冷冻术前1d外周血CTCs阳性检测率为45.0％,冷冻术后10d CTCs阳性检测率为15.0％,治疗后患者的CTCs阳性率较术前显著下降,差异有统计学意义(x2=4.286,P＜0.05).3例术后CTCs阳性患者的中位生存期为5个月,17例术后CTCs阴性患者中位生存期为8个月,CTCs阴性患者的生存期显著长于CTCs阳性患者,差异有统计学意义(x2=5.552,P=0.0185).
结论：采用MACS联合定量PCR法检测胰腺癌患者外周血CTCs,对评估行冷冻治疗胰腺癌患者的预后有一定临床价值.
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