Flow injection chemiluminescence experiments were employed to determinate the cocentration of BPO in flour brightener and investigate an optimizd flow injection chemiliuminescence detecting.Microchip was fabricated with micromolding using SU-8 photo resistor (Microchem Co.USA) and PDMS (Sylgard184, Dow Coring Co.USA).The completed microchannel is reactangle with wihth 500μm and height 40μm, respectively.Syringe and peristaltic pump were employed to inject standard BPO and luminor solutions, while concentration of the two reactors were 0.001 g mL-1 and 0.01 mol L-1, respectively.The recorded results (Fig.1) suggests that the peristaltic pump conducts less fluctuation of CL intersity with a small relative deviation of 6% in contrast to 30% of syringe pump.