Hydrogen sulfide inhibits TGF-β1 induced bronchial epithelial mesenchymal transition

来源 :第十二届中青年呼吸学者论坛 | 被引量 : 0次 | 上传用户：adward006

【摘要】

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Objective: To investigate the effects of H2S on TGF-β1-induced EMT in human bronchial epithelial cell.Methods: TGF-β1 was applied to induce EMT in 16HBE.H2Swa

【作者】

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LIAO Cheng-cheng CHEN Ya-hong LIN Fan QI Yong-fen

【机构】

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DepartmentofRespiratory,PekingUniversityThirdHospital,Beijing100191,ChinaLaboratoryofCardiovascularB

【出处】

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第十二届中青年呼吸学者论坛

【发表日期】

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2015年7期

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Objective: To investigate the effects of H2S on TGF-β1-induced EMT in human bronchial epithelial cell.Methods: TGF-β1 was applied to induce EMT in 16HBE.H2Swas pre-incubated for 1h and then followed by TGF-β1 for 72 h.EMT markers were examined by western blot.Changes in cell morphology were assessed under phase-contrast light microscopy.Wound healing assay were used to evaluate motility of cells.Results: The cells displayed an elongated shape, with less contact with each other and increased motility after treatment with TGF-β1.In contrast, H2S treatment reversed the cell abnormal morphology and motility induced by TGF-β 1.Moreover, TGF-β1 treatment significantly increased the expression of α-SMA and decreased the expression of E-cadherin.Compared with TGF-β1 group,H2S could reverse these markers by reduced the expression of α-SMA by 45.1％ and increase the expression of E-cadherin by 57.6％ (P＜0.05).Conclusion: Exogenous H2S inhibits TGF-β1-induced EMT in 16HBE.

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Hydrogen sulfide inhibits TGF-β1 induced bronchial epithelial mesenchymal transition

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