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Taking tissue-cultured seedings of Populus davidiana x P.bolleana as experimental material, through pot experiment, the effects of root application of Trichoderma asperellum ACCC305 36 conidia of T1 (5x102 cfu/cm3 soil), T2 (5x103 cfu/cm3 soil) and T3 (5x104 cfu/cm3 soil) on growth and leaf photosynthetic characters of Populus davidiana x P.bolleana seedings were studied.The results showed that T2 inducing had the best effects.On the one hand, compared to all other groups, the plant height and the stem base perimeter of T2 group reached their maximums at different sample collected time points.