Hepatocellular carcinoma is the most frequently diagnosed cancer in the world.Liver cancer stem cells (LCSCs) are considered to be the main cause of recurrence, metastasis and multi-drug resistance of hepatocellular carcinoma, so it is crucial to eradicate LCSCs for the treatment of hepatocellular carcinoma.Salinomycin is a polyether antibiotic isolated from Streptomyces Albus, has recently been shown to possess anti-tumor properties by selectively inhibiting cancer stem cells population.Studies have shown that salinomycin selectively targets and decreases CSCs in hepatocellular carcinoma cells in vitro and in vivo.