目的 :建立高效液相色谱方法同时检测人血清中全反式及 13 -顺式维甲酸浓度。方法 :色谱柱 :μBondapakC18(3 9mm× 30 0mm) ,流动相 :甲醇 -醋酸铵缓冲液 (85∶15 ) ,流速 :0 8mL·min-1,紫外检测波长 :340nm ,柱温 :2 2℃ ,血清样品经乙醚 2次提取 ,按内标法定量 ,内标物选用对二甲氨基苯甲醛。结果 :全反式及 13-顺式维甲酸的线性范围分别在 0 8～ 112 0 μg·L-1和 0 82～ 1312 μg·L-1,最低检测浓度均为 0 6μg·L-1。全反式维甲酸测定的平均回收率为 98 86 %～ 10 5 2 % ,日内、日间精密度RSD为 0 84%～ 5 5 % ,13-顺式维甲酸测定的平均回收率为 10 1 6 %～ 10 1 8% ,日内、日间精密度RSD为 1 4%～ 5 7%。结论 :本方法灵敏、准确 ,样品处理简便易行 ,适用于全反式维甲酸的临床药动学研究 Objective: To establish a high performance liquid chromatographic method for the simultaneous determination of all - trans and 13 - cis retinoic acid in human serum. Methods: The chromatographic column was μBondapak C18 (39 mm × 30 0 mm). The mobile phase consisted of methanol-ammonium acetate buffer (85:15), flow rate of 0 8 mL · min-1, UV detection wavelength of 340 nm and column temperature of 22 ℃ , Serum samples were extracted twice with diethyl ether, according to the internal standard method, the internal standard selection of p-dimethylaminobenzaldehyde. Results: The linear ranges of all-trans and 13-cis retinoid were 0 8-1 12 0 μg · L-1 and 0 82-1 13 2 μg · L-1, respectively. The lowest detection concentration was 0 6 μg · L-1. The average recovery of all-trans retinoic acid was 98 86% ~ 105 2%. The intra-day and inter-day RSD was 0 84% ~ 55%. The average recovery of 13-cis retinoic acid was 10 1 6% -101 8%. The intra-day and inter-day RSD was 14% ~ 57%. Conclusion: The method is sensitive and accurate, the sample processing is simple and easy, and it is suitable for clinical pharmacokinetic study of all-trans retinoic acid